Rapid analysis of pregnanediol in pregnancy urine.
نویسندگان
چکیده
A simple, inexpensive, and rapid method for the determination of pregnanediol in pregnancy urine by gas liquid chromatography is described. Automatic injection of the samples into the gas chromatograph allows up to 36 samples to be run overnight thus saving valuable technical time.The method described can easily be adopted for use in a routine steroid laboratory. The results obtained by this method have been compared with values obtained by the method of Klopper, Michie, and Brown (1955).
منابع مشابه
Rapid gas chromatographic assay for pregnanolone in pregnancy urine.
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Venning and Browne (1 to 7) have shown that sodium pregnanediol glucuronidate is excreted in urine of human pregnancy in concentrations which become increasingly higher as gestation approaches term. Although this demonstration has been repeatedly confirmed (8 to 16), relatively few workers have studied the precise levels and ranges of normal excretion, or have secured long-continued records of ...
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It has recently been shown (Marrian & Gough, 1946 a, b) that the 'sodium pregnanediol glucuronidate' ('NaPG') prepared from human pregnancy urine by the method of Venning & Browne (1936) contains about 20% of a water-soluble derivative of pregnan-3(o)-ol-20-one which cannot be removed by repeated recrystallization from 90% (v/v) aqueous ethanol or by repeated precipitation from 50% (v/v) aqueou...
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عنوان ژورنال:
- Journal of clinical pathology
دوره 25 11 شماره
صفحات -
تاریخ انتشار 1972